Thiol quantitation assays and related methods

ABSTRACT

The present invention is generally directed to thiol quantitation assays, methods of performing the assays, and compounds used in the assays. It is more specifically directed to assays that include one or more disulfides and related molecules and methods. The disulfides contain a FRET pair.

CROSS-REFERENCE TO RELATED APPLICATION

This application claims priority to U.S. Provisional Patent Application Ser. No. 61/273,186, filed on Jul. 30, 2009, the entire disclosure of which is incorporated by reference.

FIELD OF THE INVENTION

The present invention is generally directed to thiol quantitation assays, methods of performing the assays, and compounds used in the assays. It is more specifically directed to assays that include one or more disulfides and related molecules and methods.

BACKGROUND OF THE INVENTION

Thiols play a number of important roles in cellular biochemistry. In certain cases they determine protein structure; in others they serve as covalent catalysts; in still others they maintain appropriate oxidation states of proteins and cells. Accordingly, thiol detection and quantitation is important to understanding cellular processes.

Several assays have been developed for thiol quantitation. One such assay is the Thiol and Sulfide Quantitation Kit commercialized by Invitrogen. The kit includes the following: Papain-SSCH₃, a disulfide-inhibited papain derivative; L-BAPNA, a chromogenic papain substrate; DTNB (Ellman's reagent) for calibrating the assay; cystamine; L-cysteine, a thiol standard; and a buffer. According to product literature, the chemical basis for the assay involves the following reactions: 1) Papain-SSCH₃ is activated in the presence of thiols. 2) Active papain cleaves the substrate L-BAPNA, which releases the p-nitroaniline chromophore. 3) Protein thiols exchange with cystamine to generate 2-mercaptoethylamine, which is detected.

Another thiol quantitation assay has been worked on at Marquette University by Sem and Pullela. See U.S. patent application Ser. No. 11/512,485. Their work focuses on hydroxyl-coumarin-based disulfides, which reportedly react with sulfides present in an assay well. In a related publication the researches noted it was difficult to find a molecule that worked effectively in an assay framework. See, The FASEB Journal, 2008;22:1059.3.

Despite the efforts discussed above, there is still a need for novel thiol quantitation assays that meet the unmet needs of various researchers.

SUMMARY OF THE INVENTION

The present invention is generally directed to thiol quantitation assays, methods of performing the assays, and compounds used in the assays. It is more specifically directed to assays that include one or more disulfides and related molecules and methods.

In a method aspect, the present invention provides a method of quantifying a thiol in a sample. The method comprises the steps of: a) contacting the sample with a disulfide; and, b) detecting fluorescence from a cleavage product of the disulfide after the cleavage product has been excited. The disulfide is of the following structure:

-   wherein R₁ is OH or NH₂, R₂ is H or SO₃—, R₃ is H or SO₃—, and R₄ is     NH₂+ or O; and -   wherein X—Y is a symmetrical or unsymmetrical disulfide having the     general structure —NH—R₅—SS—R₆—NH—, where R₅ and R₆ are either aryl     groups or alkyl groups.

In a compound aspect, the present invention provides a disulfide of the following structure:

-   wherein R₁ is OH or NH₂, R₂ is H or SO₃—, R₃ is H or SO₃—, and R₄ is     NH₂+ or O; and -   wherein X—Y is a symmetrical or unsymmetrical disulfide having the     general structure —NH—R₅—SS—R₆—NH—, where R₅ and R₆ are either aryl     groups or alkyl groups.

DETAILED DESCRIPTION OF THE INVENTION

The present assays include one or more disulfide compounds. The disulfides contain a donor-acceptor FRET pair. When the disulfide is added to an assay medium, thiols present in the medium cleave the disulfide. This allows the emission spectrum of the donor portion of the pair to be observed; a thiol is detected and can be quantified according to the intensity of the emission.

Disulfides according to the present invention have a general structure as shown below (Structure I):

-   R₁ is OH or NH; R₂ is H or SO₃—; R₃ is H or SO₃—; and R₄ is O or     NH₂+. “X—Y” is a symmetrical or unsymmetrical disulfide having the     general structure —NH—R₅—SS—R₆—NH—, where R₅ and R₆ are either aryl     groups or alkyl groups.

Structure II and Structure III below depict two more specific structures of disulfides of the present invention, where the substitution pattern on one aryl moiety is defined. The substituents for these structures are the same as for Structure I.

Structure IV and Structure V below depict two still more specific structures of disulfides of the present invention, where the substitution pattern on one aryl moiety and the tricyclic moiety are defined. Substituents “X—Y” for these structures are the same as for Structure I.

As noted above, the group “X—Y” is a symmetrical or unsymmetrical disulfide having the general structure —NH—R₅—SS—R₆—NH—, where R₅ and R₆ are either aryl groups or alkyl groups. The aryl groups can be unsubstituted aryl groups, substituted aryl groups, unsubstituted heteroaryl groups and substituted heteroaryl groups. An unsubstituted aryl group is represented by Structure VI, Structure VII or Structure VIII below.

A substituted aryl group is represented by Structure IX, Structure X or Structure XI below.

-   R₇, R₈, R₉ and R₁₀ are independently selected from the group     consisting of H, CH₃, CH₂CH₃, CH₂CH₂CH₃, CH(CH₃)₂, F, Cl, Br, I, CN,     OCH₃, OCH₂CH₃, CO₂CH₃, CO₂CH₂CH₃, N(CH₃)₂, N(CH₂CH₃)₂, SCH₃,     SCH₂CH₃; at least one of the substituents is not H.

An unsubstituted heteroaryl group is represented by Structure XII, Structure XIII, Structure XIV, Structure XV, Structure XVI, Structure XVII, Structure XVIII, Structure XIX and Structure XX below.

A substituted heteroaryl group is represented by Structure XXI, Structure XXII, Structure XXIII, Structure XXIV, Structure XXV, Structure XXVI, Structure XXVII, Structure XXVIII and Structure XXIX below.

-   R₁₁ and R₁₂ are independently selected from the group consisting of     H, CH₃, CH₂CH₃, CH₂CH₂CH₃, CH(CH₃)₂, F, Cl, Br, I, CN, OCH₃,     OCH₂CH₃, CO₂CH₃, CO₂CH₂CH₃, N(CH₃)₂, N(CH₂CH₃)₂, SCH₃, SCH₂CH₃; at     least one of the substituents is not H.

-   R₁₃ and R₁₄ are independently selected from the group consisting of     H, CH₃, CH₂CH₃, CH₂CH₂CH₃, CH(CH₃)₂, F, Cl, Br, I, CN, OCH₃,     OCH₂CH₃, CO₂CH₃, CO₂CH₂CH₃, N(CH₃)₂, N(CH₂CH₃)₂, SCH₃, SCH₂CH₃; at     least one of the substituents is not H.

-   R₁₅ and R₁₆ are independently selected from the group consisting of     H, CH₃, CH₂CH₃, CH₂CH₂CH₃, CH(CH₃)₂, F, Cl, Br, I, CN, OCH₃,     OCH₂CH₃, CO₂CH₃, CO₂CH₂CH₃, N(CH₃)₂, N(CH₂CH₃)₂, SCH₃, SCH₂CH₃; at     least one of the substituents is not H.

Where R₅ and/or R₆ are alkyl groups, the alkyl groups can be unsubstituted alkyl groups, substituted alkyl groups and heteroalkyl groups. The following are non-limiting examples of unsubstituted alkyl groups: —CH₂CH₂—; —CH₂CH₂CH₂—; —CH(CH₃)CH₂—; —CH(CH₃)CH₂CH₂—; —CH₂CH(CH₃)CH₂—. The following are non-limiting examples of substituted alkyl groups: —CH(CO₂CH₃)CH₂—; —CH(CO₂CH₂CH₃)CH₂—; —CH₂CH(OCH₃)CH₂—; —CH₂CH(CN)CH₂—; —CH₂CH(CO₂CH₃)CH₂—; —CH₂CH(CH₂CO₂CH₃)CH₂—; —CH₂CH(OCH₃)CH₂CH₂—. The following are non-limiting examples of heteroalkyl groups: —CH₂CH₂—O—CH₂CH₂—; —CH₂CH₂—N[C(O)CH₃]—CH₂CH₂—; —CH₂CH₂—S—CH₂CH₂—.

The group “X—Y” is symmetrical or asymmetrical. Non-limiting examples of such groups are: —NH—CH₂CH₂—SS—CH₂CH₂—NH—; —NH—(C₆H₄)—SS—(C₆H₄)—NH—; —NH—CH₂CH₂—SS—(C₆H₄)—NH—; —NH—(C₆H₄)—SS—CH₂CH₂—NH—; —NH—CH₂CH₂—SS—CH₂CH₂CH₂—NH—; —NH—CH₂CH₂CH₂—SS—CH₂CH₂—NH—; —NH—CH₂CH₂CH₂—SS—CH₂CH₂CH₂—NH—; —NH—CH₂CH₂CH₂CH₂—SS—CH₂CH₂—NH—; —NH—CH₂CH₂CH₂CH₂—SS—CH₂CH₂CH₂—NH—; —NH—CH₂CH₂CH₂CH₂—SS—CH₂CH₂CH₂CH₂—NH—; —NH—CH₂CH₂₋OCH₂CH₂—SS—CH₂CH₂—NH—; —NH—CH₂CH₂OCH₂CH₂—SS—CH₂CH₂CH₂—NH—; —NH—CH₂CH₂OCH₂CH₂—SS—CH₂CH₂CH₂CH₂—NH—; —NH—CH₂CH₂OCH₂CH₂—SS—CH₂CH₂OCH₂CH₂—NH—; —NH—CH₂CH₂—SS—CH₂CH₂OCH₂CH₂—NH—; —NHCH₂CH₂CH₂—SS—CH₂CH₂OCH₂CH₂NH—; —NHCH₂CH₂CH₂CH₂—SS—CH₂CH₂OCH₂CH₂NH—; —NH—CH₂CH₂—SS—(C₄H₂O)—NH—; —NH—CH₂CH₂CH₂—SS—(C₄H₂O)—NH—; —NH—CH₂CH₂CH₂CH₂—SS—(C₄H₂O)—NH—; —NH—CH₂CH₂OCH₂CH₂—SS—(C₄H₂O)—NH—; —NH—(C₄H₂O)—SS—CH₂CH₂—NH—; —NH—(C₄H₂O)—SS—CH₂CH₂CH₂—NH—; —NH—(C₄H₂O)—SS—CH₂CH₂CH₂CH₂—NH—; —NH—(C₄H₂O)—SS—CH₂CH₂OCH₂CH₂—NH—; —NH(C₄H₂O)—SS—(C₄H₂O)—NH—; —NH—CH₂CH₂—SS—(C₄H₂S)—NH—; —NH—CH₂CH₂CH₂—SS—(C₄H₂S)—NH—; —NH—CH₂CH₂CH₂CH₂—SS—(C₄H₂S)—NH—; —NH—CH₂CH₂OCH₂CH₂—SS—(C₄H₂S)—NH—; —NH—(C₄H₂S)—SS—CH₂CH₂—NH—; —NH—(C₄H₂S)—SS—CH₂CH₂CH₂—NH—; —NH—(C₄H₂S)—SS—CH₂CH₂CH₂CH₂—NH—; —NH—(C₄H₂S)—SS—CH₂CH₂OCH₂CH₂—NH—; —NH(C₄H₂S)—SS—(C₄H₂S)—NH—.

Referring to Structure II, the following are non-limiting examples of disulfides according to the present invention:

EXAMPLE 1 R₁ is NH₂; R₂ is SO₃—; R₃ is SO₃—; R₄ is NH₂+; X—Y is —NH—CH₂CH₂—SS—CH₂CH₂—NH—. EXAMPLE 2 R₁ is NH₂; R₂ is SO₃—; R₃ is SO₃—; R₄ is NH₂+; X—Y is —NH-(pC₆H₄)—SS-(pC₆H₄)—NH—. EXAMPLE 3 R₁ is NH₂; R₂ is SO₃—; R₃ is SO₃—; R₄ is NH₂+; X—Y is —NH—CH₂CH₂—SS—CH₂CH₂CH₂—NH—. EXAMPLE 4 R₁ is NH₂; R₂ is SO₃—; R₃ is SO₃—; R₄ is NH₂+; X—Y is —NH—CH₂CH₂CH₂—SS—CH₂CH₂—NH—. EXAMPLE 5 R₁ is NH₂; R₂ is SO₃—; R₃ is SO₃—; R₄ is NH₂+; X—Y is —NH—CH₂CH₂—SS-(pC₆H₄)—NH—. EXAMPLE 6 R₁ is NH₂; R₂ is SO₃—; R₃ is SO₃—; R₄ is NH₂+; X—Y is —NH-(pC₆H₄)—SS—CH₂CH₂—NH—. EXAMPLE 7 R₁ is OH; R₂ is H; R₃ is H; R₄ is O; XY is —NHCH₂CH₂—SS—CH₂CH₂—NH—. EXAMPLE 8 R₁ is OH; R₂ is H; R₃ is H; R₄ is O; XY is —NH-(pC₆H₄)—SS—(pC₆H₄)—NH—. EXAMPLE 9 R₁ is OH; R₂ is H; R₃ is H; R₄ is O; XY is —NH—CH₂CH₂—SS—CH₂CH₂CH₂—NH—. EXAMPLE 10 R₁ is OH; R₂ is H; R₃ is H; R₄ is O; XY is —NH—CH₂CH₂CH₂—SS—CH₂CH₂—NH—. EXAMPLE 11 R₁ is OH; R₂ is H; R₃ is H; R₄ is O; XY is —NH—CH₂CH₂—SS—(pC6H4)—NH—. EXAMPLE 12 R₁ is OH; R₂ is H; R₃ is H; R₄ is O; XY is —NH-(pC₆H₄)—SS—CH₂CH₂—NH—.

Referring to Structure III, the following are non-limiting examples of disulfides according to the present invention:

EXAMPLE 13 R₁ is NH₂; R₂ is SO₃—; R₃ is SO₃—; R₄ is NH₂+; X—Y is —NH—CH₂CH₂—SS—CH₂CH₂—NH—. EXAMPLE 14 R₁ is NH₂; R₂ is SO₃—; R₃ is SO₃—; R₄ is NH₂+; X—Y is —NH-(pC₆H₄)—SS—(pC₆H₄)—NH—. EXAMPLE 15 R₁ is NH₂; R₂ is SO₃—; R₃ is SO₃—; R₄ is NH₂+; X—Y is —NH—CH₂CH₂—SS—CH₂CH₂CH₂—NH—. EXAMPLE 16 R₁ is NH₂; R₂ is SO₃—; R₃ is SO₃—; R₄ is NH₂+; X—Y is —NH—CH₂CH₂CH₂—SS—CH₂CH₂—NH—. EXAMPLE 17 R₁ is NH₂; R₂ is SO₃—; R₃ is SO₃—; R₄ is NH₂+; X—Y is —NH—CH₂CH₂—SS—(pC₆H₄)—NH—. EXAMPLE 18 R₁ is NH₂; R₂ is SO₃—; R₃ is SO₃—; R₄ is NH₂+; X—Y is —NH-(pC₆H₄)—SS—CH₂CH₂—NH—. EXAMPLE 19 R₁ is OH; R₂ is H; R₃ is H; R₄ is O; XY is —NHCH₂CH₂—SS—CH₂CH₂—NH—. EXAMPLE 20 R₁ is OH; R₂ is H; R₃ is H; R₄ is O; XY is —NH-(pC₆H₄)—SS—(pC₆H₄)—NH—. EXAMPLE 21 R₁ is OH; R₂ is H; R₃ is H; R₄ is O; XY is —NH—CH₂CH₂—SS—CH₂CH₂CH₂—NH—. EXAMPLE 22 R₁ is OH; R₂ is H; R₃ is H; R₄ is O; XY is —NH—CH₂CH₂CH₂—SS—CH₂CH₂—NH—. EXAMPLE 23 R₁ is OH; R₂ is H; R₃ is H; R₄ is O; XY is —NH—CH₂CH₂—SS-(pC₆H₄)—NH—. EXAMPLE 24 R₁ is OH; R₂ is H; R₃ is H; R₄ is O; XY is —NH-(pC₆H₄)—SS—CH₂CH₂—NH—.

The disulfides of the present invention are synthesized according to methods known to those skilled in the art. Examples of reaction types that are used to synthesize the disulfides can be found in U.S. patent application Ser. No. 11/512,485, which is incorporated-by-reference into this document for all purposes. One such reaction type is the condensation of a diamine (e.g., H₂N—CH₂CH₂—SS—CH₂CH₂—NH₂ and H₂N—(C₆H₄)—SS—(C₆H₄)—NH₂) with an activated carboxylic acid moiety of a FRET pair donor and a FRET pair acceptor, which is typically performed in two separate synthetic steps.

Assays of the present invention are performed by bringing a disulfide of the present invention in contact with a sample thought to include one or more types of thiols. As noted above, the disulfides contain a donor-acceptor FRET pair. When the disulfide is added to the sample, thiols present in the sample cleave the disulfide. Cleavage ensures that the acceptor no longer quenches the emission spectrum of the donor portion of the FRET pair. Excitation of the donor accordingly results in fluorescence, which can be measured. 

The invention claimed is:
 1. A method of quantifying a thiol in a sample, wherein the method comprises the steps of: a) contacting the sample with a disulfide, where the disulfide is of the following structure

wherein R₁ is OH or NH₂, R₂ is H or SO₃—, R₃ is H or SO₃—, and R₄ is NH₂+ or O; and wherein X—Y is a symmetrical or unsymmetrical disulfide having the general structure —NH—R₅—SS—R₆—NH—, where R₅ and R₆ are either aryl groups or alkyl groups; b) detecting fluorescence from a cleavage product of the disulfide after the cleavage product has been excited.
 2. The method according to claim 1, wherein the disulfide is of the following structure:


3. The method according to claim 2, wherein R₁ is NH₂, R₂ is SO₃—, R₃ is SO₃—, and R₄ is NH₂+.
 4. The method according to claim 3, wherein X—Y is selected from a group consisting of —NH—CH₂CH₂—SS—CH₂CH₂—NH—; —NH—(C₆H₄)—SS—(C₆H₄)—NH—; —NHCH₂CH₂—SS—(C₆H₄)—NH—; —NH—(C₆H₄)—SS—CH₂CH₂—NH—; —NHCH₂CH₂—SS—CH₂CH₂CH₂—NH—; —NHCH₂CH₂—SS—(C₄H₂O)—NH—; and —NH(C₄H₂O)—SS—(C₄H₂O)—NH—.
 5. The method according to claim 2, wherein R₁ is OH, R₂ is H, R₃ is H, and R₄ is O.
 6. The method according to claim 5, wherein X—Y is selected from a group consisting of —NH—CH₂CH₂—SS—CH₂CH₂—NH—; —NH—(C₆H₄)—SS—(C₆H₄)—NH—; —NHCH₂CH₂—SS—(C₆H₄)—NH—; —NH—(C₆H₄)—SS—CH₂CH₂—NH—; —NHCH₂CH₂—SS—CH₂CH₂CH₂—NH—; —NHCH₂CH₂—SS—(C₄H₂O)—NH—; and —NH(C₄H₂O)—SS—(C₄H₂O)—NH—.
 7. The method according to claim 1, wherein the disulfide is of the following structure:


8. The method according to claim 7, wherein R₁ is NH₂, R₂ is SO₃—, R₃ is SO₃—, and R₄ is NH₂+.
 9. The method according to claim 8, wherein X—Y is selected from a group consisting of —NH—CH₂CH₂—SS—CH₂CH₂—NH—; —NH—(C₆H₄)—SS—(C₆H₄)—NH—; —NHCH₂CH₂—SS—(C₆H₄)—NH—; —NH—(C₆H₄)—SS—CH₂CH₂—NH—; —NHCH₂CH₂—SS—CH₂CH₂CH₂—NH—; —NHCH₂CH₂—SS—(C₄H₂O)—NH—; and —NH(C₄H₂O)—SS—(C₄H₂O)—NH—.
 10. The method according to claim 7, wherein R₁ is OH, R₂ is H, R₃ is H, and R₄ is O.
 11. The method according to claim 10, wherein X—Y is selected from a group consisting of —NH—CH₂CH₂—SS—CH₂CH₂—NH—; —NH—(C₆H₄)—SS—(C₆H₄)—NH—; —NHCH₂CH₂—SS—(C₆H₄)—NH—; —NH—(C₆H₄)—SS—CH₂CH₂—NH—; —NHCH₂CH₂—SS—CH₂CH₂CH₂—NH—; —NHCH₂CH₂—SS—(C₄H₂O)—NH—; and —NH(C₄H₂O)—SS—(C₄H₂O)—NH—.
 12. A compound, wherein the compound is of the following structure:

wherein R₁ is OH or NH₂, R₂ is H or SO₃—, R₃ is H or SO₃—, and R₄ is NH₂+ or O; and wherein X—Y is a symmetrical or unsymmetrical disulfide having the general structure —NH—R₅—SS—R₆—NH—, where R₅ and R₆ are either aryl groups or alkyl groups.
 13. The compound according to claim 12, wherein the compound is of the following structure:


14. The compound according to claim 13, wherein R₁ is NH₂, R₂ is SO₃—, R₃ is SO₃—, and R₄ is NH₂+.
 15. The compound according to claim 14, wherein X—Y is selected from a group consisting of —NH—CH₂CH₂—SS—CH₂CH₂—NH—; —NH—(C₆H₄)—SS—(C₆H₄)—NH—; —NHCH₂CH₂—SS—(C₆H₄)—NH—; —NH—(C₆H₄)—SS—CH₂CH₂—NH—; —NHCH₂CH₂—SS—CH₂CH₂CH₂—NH—; —NHCH₂CH₂—SS—(C₄H₂O)—NH—; and —NH(C₄H₂O)—SS—(C₄H₂O)—NH—.
 16. The compound according to claim 13, wherein R₁ is OH, R₂ is H, R₃ is H, and R₄ is O.
 17. The compound according to claim 16, wherein X—Y is selected from a group consisting of —NH—CH₂CH₂—SS—CH₂CH₂—NH—; —NH—(C₆H₄)—SS—(C₆H₄)—NH—; —NHCH₂CH₂—SS—(C₆H₄)—NH—; —NH—(C₆H₄)—SS—CH₂CH₂—NH—; —NHCH₂CH₂—SS—CH₂CH₂CH₂—NH—; —NHCH₂CH₂—SS—(C₄H₂O)—NH—; and —NH(C₄H₂O)—SS—(C₄H₂O)—NH—.
 18. The compound according to claim 12, wherein the compound is of the following structure:


19. The compound according to claim 18, wherein R₁ is NH₂, R₂ is SO₃—, R₃ is SO₃—, and R₄ is NH₂+.
 20. The compound according to claim 18, wherein X—Y is selected from a group consisting of —NH—CH₂CH₂—SS—CH₂CH₂—NH—; —NH—(C₆H₄)—SS—(C₆H₄)—NH—; —NHCH₂CH₂—SS—(C₆H₄)—NH—; —NH—(C₆H₄)—SS—CH₂CH₂—NH—; —NHCH₂CH₂—SS—CH₂CH₂CH₂—NH—; —NHCH₂CH₂—SS—(C₄H₂O)—NH—; and —NH(C₄H₂O)—SS—(C₄H₂O)—NH—.
 21. The compound according to claim 18, wherein R₁ is OH, R₂ is H, R₃ is H, and R₄ is O.
 22. The compound according to claim 21, wherein X—Y is selected from a group consisting of —NH—CH₂CH₂—SS—CH₂CH₂—NH—; —NH—(C₆H₄)—SS—(C₆H₄)—NH—; —NHCH₂CH₂—SS—(C₆H₄)—NH—; —NH—(C₆H₄)—SS—CH₂CH₂—NH—; —NHCH₂CH₂—SS—CH₂CH₂CH₂—NH—; —NHCH₂CH₂—SS—(C₄H₂O)—NH—; and —NH(C₄H₂O)—SS—(C₄H₂O)—NH—. 